| Trail Formation Alleviates Excessive Adhesion and Maintains Efficient Neutrophil Migration | |
Hu WH(胡文慧); Gao WB(高文博); Gong YX(龚一心) ; Guo P(郭盼); Li W(李旺); Shu XY(舒芯钰); Lv SQ(吕守芹); Zeng, Zhu2; Zhang Y(章燕); Long M(龙勉)
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| Source Publication | ACS APPLIED MATERIALS & INTERFACES
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| 2023 | |
| ISSN | 1944-8244 |
| Abstract | Migrating neutrophils are found to leave behind subcellular trails in vivo, but the underlying mechanisms remain unclear. Here, an in vitro cell migration test plus an in vivo observation was applied to monitor neutrophil migration on intercellular cell adhesion molecule 1 (ICAM 1) presenting surfaces. Results indicated that migrating neutrophils left behind long lasting, chemokine containing trails. Trail formation tended to alleviate excessive cell adhesion enhanced by the trans binding antibody and maintain efficient cell migration, which was associated with differential instantaneous edge velocity between the cell front and rear. CD11a and CD11b worked differently in inducing trail formation with polarized distributions on the cell body and uropod. Trail release at the cell rear was attributed to membrane ripping, in which fi2 integrin was disrupted from the cell membrane through myosin mediated rear contraction and integrin cytoskeleton dissociation, potentiating a specialized strategy of integrin loss and cell deadhesion to maintain efficient migration. Moreover, neutrophil trails left on the substrate served as immune forerunners to recruit dendritic cells. These results provided an insight in elucidating the mechanisms of neutrophil trail formation and deciphering the roles of trail formation in efficient neutrophil migration. |
| Keyword | neutrophil migration trail release rear contraction fi2 integrin cell adhesion |
| DOI | 10.1021/acsami.3c00288 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000962907300001 |
| WOS Research Area | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| WOS Subject | Science & Technology Other Topics ; Materials Science |
| Funding Organization | National Natural Science Foundation of China [32130061, 12272389, 11772345] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences [GJJSTU20220002] ; Frontier Science Key Project of Chinese Science Academy [QYZDJ SSW JSC018] |
| Classification | 一类 |
| Ranking | 1 |
| Contributor | Zhang, Y ; Long, M (corresponding author), Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China. ; Zhang, Y ; Long, M (corresponding author), Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construction & Mechanobiol, Beijing 100190, Peoples R China. ; Zhang, Y ; Long, M (corresponding author), Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China. |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/91875 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construction & Mechanobiol, Beijing 100190, Peoples R China 3.Guizhou Med Univ, Sch Basic Med Sci, Guiyang 550025, Peoples R China 4.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Hu WH,Gao WB,Gong YX,et al. Trail Formation Alleviates Excessive Adhesion and Maintains Efficient Neutrophil Migration[J]. ACS APPLIED MATERIALS & INTERFACES,2023.Rp_Au:Zhang, Y, Long, M (corresponding author), Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China., Zhang, Y, Long, M (corresponding author), Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construction & Mechanobiol, Beijing 100190, Peoples R China., Zhang, Y, Long, M (corresponding author), Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China. |
| APA | 胡文慧.,高文博.,龚一心.,郭盼.,李旺.,...&龙勉.(2023).Trail Formation Alleviates Excessive Adhesion and Maintains Efficient Neutrophil Migration.ACS APPLIED MATERIALS & INTERFACES. |
| MLA | 胡文慧,et al."Trail Formation Alleviates Excessive Adhesion and Maintains Efficient Neutrophil Migration".ACS APPLIED MATERIALS & INTERFACES (2023). |
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| File Name/Size | DocType | Version | Access | License | ||
| Jp2023Fa291.pdf（7494KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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