| Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion | |
Jiang, Chao; Luo, Hong-Yu; Xu, Xinpeng; Dou, Shuo-Xing; Li, Wei; Guan DS(关东石); Ye, Fangfu; Chen, Xiaosong; Guo, Ming; Wang, Peng-Ye; Li, Hui
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| Corresponding Author | Wang, Peng-Ye([email protected]) ; Li, Hui([email protected]) |
| Source Publication | NATURE COMMUNICATIONS
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| 2023-08-24 | |
| Volume | 14Issue:1Pages:13 |
| Abstract | Cell migration plays important roles in many biological processes, but how migrating cells orchestrate intracellular molecules and subcellular structures to regulate their speed and direction is still not clear. Here, by characterizing the intracellular diffusion and the three-dimensional lamellipodium structures of fish keratocyte cells, we observe a strong positive correlation between the intracellular diffusion and cell migration speed and, more importantly, discover a switching of cell migration modes with reversible intracellular diffusion variation and lamellipodium structure deformation. Distinct from the normal fast mode, cells migrating in the newly-found slow mode have a deformed lamellipodium with swollen-up front and thinned-down rear, reduced intracellular diffusion and compartmentalized macromolecule distribution in the lamellipodium. Furthermore, in turning cells, both lamellipodium structure and intracellular diffusion dynamics are also changed, with left-right symmetry breaking. We propose a mechanism involving the front-localized actin polymerization and increased molecular crowding in the lamellipodium to explain how cells spatiotemporally coordinate the intracellular diffusion dynamics and the lamellipodium structure in regulating their migrations. |
| DOI | 10.1038/s41467-023-40858-x |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001054501000003 |
| WOS Keyword | PARTICLE TRACKING ; ACTIN ; CYTOPLASM ; FLOW ; MECHANICS ; DYNAMICS ; MOTILITY ; DRIVEN ; SHAPE ; TRANSPORT |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| Funding Project | We thank National Center for Protein Sciences at Peking University, particularly Dr. Siying Qin, for technical help with AFM. We thank Dr. Qihui Fan, Dr. Ruipei Xie, and Yuhang Li for their technical help with micromanipulation. We thank Optofem Technology[12074043] ; We thank National Center for Protein Sciences at Peking University, particularly Dr. Siying Qin, for technical help with AFM. We thank Dr. Qihui Fan, Dr. Ruipei Xie, and Yuhang Li for their technical help with micromanipulation. We thank Optofem Technology[11874415] ; We thank National Center for Protein Sciences at Peking University, particularly Dr. Siying Qin, for technical help with AFM. We thank Dr. Qihui Fan, Dr. Ruipei Xie, and Yuhang Li for their technical help with micromanipulation. We thank Optofem Technology[12135003] ; National Natural Science Foundation of China ; [12122402] |
| Funding Organization | We thank National Center for Protein Sciences at Peking University, particularly Dr. Siying Qin, for technical help with AFM. We thank Dr. Qihui Fan, Dr. Ruipei Xie, and Yuhang Li for their technical help with micromanipulation. We thank Optofem Technology ; National Natural Science Foundation of China |
| Classification | 一类 |
| Ranking | 3+ |
| Contributor | Wang, Peng-Ye ; Li, Hui |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/93335 |
| Collection | 非线性力学国家重点实验室 |
| Recommended Citation GB/T 7714 | Jiang, Chao,Luo, Hong-Yu,Xu, Xinpeng,et al. Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion[J]. NATURE COMMUNICATIONS,2023,14,1,:13.Rp_Au:Wang, Peng-Ye, Li, Hui |
| APA | Jiang, Chao.,Luo, Hong-Yu.,Xu, Xinpeng.,Dou, Shuo-Xing.,Li, Wei.,...&Li, Hui.(2023).Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion.NATURE COMMUNICATIONS,14(1),13. |
| MLA | Jiang, Chao,et al."Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion".NATURE COMMUNICATIONS 14.1(2023):13. |
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| Jp2023A036.pdf（4603KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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