Apparent isotope effects of reversible enzymatic reactions

doi:10.1016/j.gca.2024.01.021

	Apparent isotope effects of reversible enzymatic reactions
	He YY(何雨旸); Thullner, Martin; Qin, Rui; Pearson, Ann
通讯作者	He, Yuyang([email protected])
发表期刊	GEOCHIMICA ET COSMOCHIMICA ACTA
	2024-04-01
卷号	370 页码:188-198
ISSN	0016-7037
摘要	Accurately predicting apparent isotope effects of biogeochemical processes can aid the interpretation of laboratory batch experiments and field observations. It has been observed that the apparent instantaneous isotope fractionation factor changes with residual substrate concentration during a reaction process. The change can be attributed to the reversibility of a biogeochemical reaction, which is often overlooked for convenience. Here, we explore the influence of reverse reactions on high -dimensional isotope effects in the framework of Reverse Michaelis-Menten (RMM) kinetics. The apparent instantaneous isotope fractionation factors, as well as the isotope fractionation relationships between two elements of the same nonlabile compound in the RMM model, are functions of specific affinities of the product and substrate to an enzyme and the product -substrate ratios for both isotopes. In our cases, the influence of reaction reversibility is negligible when the substrate consumption rate is less than 63%. The analysis of the simultaneous stable isotope fractionation of two elements in a compound and of the relationship between the associated isotope fractionation factors can serve as a more effective indicator for characterizing the reaction mechanism.
关键词	Rayleigh Distillation Model Isotope fractionation factor Reverse Michaelis-Menten kinetics Bonded isotope effect Batch experiment
DOI	10.1016/j.gca.2024.01.021
收录类别	SCI
语种	英语
WOS记录号	WOS:001223315400001
关键词[WOS]	DISSIMILATORY SULFATE REDUCTION ; BIOAVAILABILITY RESTRICTIONS ; NITROGEN LIMITATION ; NITRATE REDUCTION ; FRACTIONATION ; SULFUR ; OXYGEN ; KINETICS ; DENITRIFICATION ; EXCHANGE
WOS研究方向	Geochemistry & Geophysics
WOS类目	Geochemistry & Geophysics
资助项目	National Science Foundation of China (NSFC)[42150202] ; National Science Foundation of China (NSFC)[42173041] ; Key Research Program of the Institute of Geology and Geophysics, Chinese Academy of Sciences[IGGCAS-202204]
项目资助者	National Science Foundation of China (NSFC) ; Key Research Program of the Institute of Geology and Geophysics, Chinese Academy of Sciences
论文分区	一类
力学所作者排名	1
RpAuthor	He, Yuyang
引用统计
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/95260
专题	高温气体动力学国家重点实验室
推荐引用方式 GB/T 7714	He YY,Thullner, Martin,Qin, Rui,et al. Apparent isotope effects of reversible enzymatic reactions[J]. GEOCHIMICA ET COSMOCHIMICA ACTA,2024,370:188-198.Rp_Au:He, Yuyang
APA	何雨旸,Thullner, Martin,Qin, Rui,&Pearson, Ann.(2024).Apparent isotope effects of reversible enzymatic reactions.GEOCHIMICA ET COSMOCHIMICA ACTA,370,188-198.
MLA	何雨旸,et al."Apparent isotope effects of reversible enzymatic reactions".GEOCHIMICA ET COSMOCHIMICA ACTA 370(2024):188-198.