| Selecting Monoclonal Cell Lineages from Somatic Reprogramming Using Robotic-Based Spatial-Restricting Structured Flow | |
Chen, Xueping1; Fan, Ke1; Lu, Jun1,7; Zhang, Sheng1; Dong, Jianhua1; Qin, Jisheng1; Fan, Weihua1; Wang, Yan1; Zhang, Yiyuan1; Peng, Huo1; Zhang, Zhizhong1; Sun, Zhiyong1; Yu, Chunlai8; Xiong, Yucui1; Song, Yan1; Ye, Qingqing1; Mai, Shiwen1; Wang, Yuanhua1; Wang, Qizheng1; Zhang, Fengxiang1; Wen, Xiaohui1; Zhou, Tiancheng1; Han, Li3; Long M(龙勉)2 ; Pan, Guangjin1; Burke, Julian F6; Zhang, Xiao1,4,5
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| Corresponding Author | Zhang, Xiao([email protected]) |
| Source Publication | RESEARCH
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| 2024-03-07 | |
| Volume | 7Pages:16 |
| ISSN | 2096-5168 |
| Abstract | Somatic cell reprogramming generates induced pluripotent stem cells (iPSCs), which serve as a crucial source of seed cells for personalized disease modeling and treatment in regenerative medicine. However, the process of reprogramming often causes substantial lineage manipulations, thereby increasing cellular heterogeneity. As a consequence, the process of harvesting monoclonal iPSCs is labor-intensive and leads to decreased reproducibility. Here, we report the first in-house developed robotic platform that uses a pin-tip-based micro-structure to manipulate radial shear flow for automated monoclonal iPSC colony selection (similar to 1 s) in a non-invasive and label-free manner, which includes tasks for somatic cell reprogramming culturing, medium changes; time-lapse-based high-content imaging; and iPSCs monoclonal colony detection, selection, and expansion. Throughput-wise, this automated robotic system can perform approximately 24 somatic cell reprogramming tasks within 50 days in parallel via a scheduling program. Moreover, thanks to a dual flow-based iPSC selection process, the purity of iPSCs was enhanced, while simultaneously eliminating the need for single-cell subcloning. These iPSCs generated via the dual processing robotic approach demonstrated a purity 3.7 times greater than that of the conventional manual methods. In addition, the automatically produced human iPSCs exhibited typical pluripotent transcriptional profiles, differentiation potential, and karyotypes. In conclusion, this robotic method could offer a promising solution for the automated isolation or purification of lineage-specific cells derived from iPSCs, thereby accelerating the development of personalized medicines. |
| DOI | 10.34133/research.0338 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001229653300001 |
| WOS Keyword | PLURIPOTENT STEM-CELL ; E-CADHERIN ; GENERATION ; MOUSE ; DIFFERENTIATION ; EXPRESSION ; SURVIVAL ; IPSCS ; OCT4 |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| Funding Project | Key Project for Instrument Development Program-Ministry of Finance[ZDYZ2012-3] ; Key Project for Instrument Development Program-Ministry of Finance[1187000169] ; Project of Instrument Development-Chinese Academy of Sciences[1187000169] ; Project of Automated Stem Cell Induction and Culture Instrument Development-Chinese Academy of Sciences[1187000170] ; Scientific Instrument Development Program-Chinese Academy of Sciences[ZDK-YYQ20210006] ; Scientific Instrument Development Program-Chinese Academy of Sciences[YJKYYQ20210042] ; Key Research Program of Chinese Academy of Sciences[O2222001] ; Guangdong Basic and Applied Basic Research Foundation[2022A1515110435] ; Guangzhou Basic and Applied Basic Research Project[2023A04J0107] ; Project funded by China Postdoctoral Science Foundation[2021M693192] ; Guangdong International Scientific Research Cooperation Project[2022A0505050037] ; Science and Technology Planning Project of Guangdong Province[2023B1212060050] |
| Funding Organization | Key Project for Instrument Development Program-Ministry of Finance ; Project of Instrument Development-Chinese Academy of Sciences ; Project of Automated Stem Cell Induction and Culture Instrument Development-Chinese Academy of Sciences ; Scientific Instrument Development Program-Chinese Academy of Sciences ; Key Research Program of Chinese Academy of Sciences ; Guangdong Basic and Applied Basic Research Foundation ; Guangzhou Basic and Applied Basic Research Project ; Project funded by China Postdoctoral Science Foundation ; Guangdong International Scientific Research Cooperation Project ; Science and Technology Planning Project of Guangdong Province |
| Classification | 一类 |
| Ranking | 3+ |
| Contributor | Zhang, Xiao |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/95415 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Peoples R China; 2.Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China; 3.Chinese Acad Sci, Inst Elect Engn, Beijing 100190, Peoples R China; 4.Guangzhou Med Univ, CAS Key Lab Regenerat Biol, Guangzhou Inst Biomed & Hlth, Joint Sch Life Sci,Chinese Acad Sci, Guangzhou 511436, Peoples R China; 5.Guangzhou Med Univ, Guangzhou 511436, Peoples R China; 6.Univ Southampton, Biol Sci, Univ Rd, Southampton SO17 1BJ, England; 7.South China Univ Technol, Sch Light Ind & Engn, Guangzhou 510641, Peoples R China; 8.Univ Elect Sci & Technol China, Chengdu 611731, Peoples R China |
| Recommended Citation GB/T 7714 | Chen, Xueping,Fan, Ke,Lu, Jun,et al. Selecting Monoclonal Cell Lineages from Somatic Reprogramming Using Robotic-Based Spatial-Restricting Structured Flow[J]. RESEARCH,2024,7:16.Rp_Au:Zhang, Xiao |
| APA | Chen, Xueping.,Fan, Ke.,Lu, Jun.,Zhang, Sheng.,Dong, Jianhua.,...&Zhang, Xiao.(2024).Selecting Monoclonal Cell Lineages from Somatic Reprogramming Using Robotic-Based Spatial-Restricting Structured Flow.RESEARCH,7,16. |
| MLA | Chen, Xueping,et al."Selecting Monoclonal Cell Lineages from Somatic Reprogramming Using Robotic-Based Spatial-Restricting Structured Flow".RESEARCH 7(2024):16. |
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