| Dynamic nanomechanical characterization of cells in exosome therapy | |
| Chen, Ye1; Zhang, Zihan2,3; Li, Ziwei2,3; Wu, Wenjie1; Lan, Shihai1; Yan, Tianhao4; Mei, Kainan1; Qiao, Zihan1; Wang , Chen1; Bai, Chuanbiao1; Li, Ziyan1; Wu, Shangquan1,5; Wang, Jianye2,3; Zhang, Qingchuan1 | |
| Corresponding Author | Wu, Shangquan([email protected]) ; Wang, Jianye([email protected]) ; Zhang, Qingchuan([email protected]) |
| Source Publication | MICROSYSTEMS & NANOENGINEERING
 |
| 2024-07-15 | |
| Volume | 10Issue:1Pages:16 |
| ISSN | 2055-7434 |
| Abstract | Exosomes derived from mesenchymal stem cells (MSCs) have been confirmed to enhance cell proliferation and improve tissue repair. Exosomes release their contents into the cytoplasmic solution of the recipient cell to mediate cell expression, which is the main pathway through which exosomes exert therapeutic effects. The corresponding process of exosome internalization mainly occurs in the early stage of treatment. However, the therapeutic effect of exosomes in the early stage remains to be further studied. We report that the three-dimensional cell traction force can intuitively reflect the ability of exosomes to enhance the cytoskeleton and cell contractility of recipient cells, serving as an effective method to characterize the therapeutic effect of exosomes. Compared with traditional biochemical methods, we can visualize the early therapeutic effect of exosomes in real time without damage by quantifying the cell traction force. Through quantitative analysis of traction forces, we found that endometrial stromal cells exhibit short-term cell roundness accompanied by greater traction force during the early stage of exosome therapy. Further experiments revealed that exosomes enhance the traction force and cytoskeleton by regulating the Rac1/RhoA signaling pathway, thereby promoting cell proliferation. This work provides an effective method for rapidly quantifying the therapeutic effects of exosomes and studying the underlying mechanisms involved. |
| DOI | 10.1038/s41378-024-00735-z |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:001271194600001 |
| WOS Keyword | UMBILICAL-CORD BLOOD ; ANGIOGENESIS ; ENDOCYTOSIS ; RETRACTION ; MECHANISM ; MIGRATION ; PROTEIN ; RHOA |
| WOS Research Area | Science & Technology - Other Topics ; Instruments & Instrumentation |
| WOS Subject | Nanoscience & Nanotechnology ; Instruments & Instrumentation |
| Funding Project | National Natural Science Foundation of China[12232017] ; National Natural Science Foundation of China[12222212] ; National Natural Science Foundation of China[12072339] ; National Science and Technology Major Project[J2019-V-0006-0100] |
| Funding Organization | National Natural Science Foundation of China ; National Science and Technology Major Project |
| Classification | 一类 |
| Ranking | 1 |
| Contributor | Wu, Shangquan ; Wang, Jianye ; Zhang, Qingchuan |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/96009 |
| Collection | 非线性力学国家重点实验室 |
| Affiliation | 1.Univ Sci & Technol China, Dept Modern Mech, CAS Key Lab Mech Behav & Design Mat, Hefei 230027, Anhui, Peoples R China; 2.Anhui Med Univ, Reprod Med Ctr, Dept Obstet & Gynecol, Affiliated Hosp 1, Hefei 230022, Peoples R China; 3.Anhui Med Univ, Anhui Prov Key Lab Reprod Hlth & Genet, Hefei 230022, Peoples R China; 4.Jilin Univ, Coll Basic Med Sci, Dept Cell Biol & Genet, Changchun 130021, Peoples R China; 5.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, 15 Beisihuan West Rd, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Chen, Ye,Zhang, Zihan,Li, Ziwei,et al. Dynamic nanomechanical characterization of cells in exosome therapy[J]. MICROSYSTEMS & NANOENGINEERING,2024,10,1,:16.Rp_Au:Wu, Shangquan, Wang, Jianye, Zhang, Qingchuan |
| APA | Chen, Ye.,Zhang, Zihan.,Li, Ziwei.,Wu, Wenjie.,Lan, Shihai.,...&Zhang, Qingchuan.(2024).Dynamic nanomechanical characterization of cells in exosome therapy.MICROSYSTEMS & NANOENGINEERING,10(1),16. |
| MLA | Chen, Ye,et al."Dynamic nanomechanical characterization of cells in exosome therapy".MICROSYSTEMS & NANOENGINEERING 10.1(2024):16. |
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