| Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation | |
Zhong SY(钟绍宇); Zheng L(郑璐); Wu Y(武亿); Sun SJ(孙树津) ; Luo, Qing; Song, Guanbin; Lv DY(吕东媛); Long M(龙勉)
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| Source Publication | BMC BIOLOGY
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| 2024-11 | |
| Volume | 22Issue:1Pages:262 |
| Abstract | BackgroundLiver organoid serves as an alternative model for liver pathophysiology in carbohydrate or lipid metabolism and xenobiotic metabolism transformation. Biomechanical cues including spaceflight mission can affect liver organoid construction and their related functions, but their underlying mechanisms are not fully understood yet. Here, a rotating cell culture device, namely Rotating Flat Chamber (RFC), was specifically designed for adhering cells or cell aggregated to elucidate the effects of altered gravity vector on HepaRG-derived liver organoids construction.ResultsThe organoids so formed under RFC presented the fast growth rate and large projection area. Meanwhile, the expressions of two pluripotency markers of SOX9 and CD44 were enhanced. This finding was positively correlated with the increased YAP expression and nuclear translocation as well as the elevated alpha 4 beta 6-integrin expression. Inhibition of YAP expression and nuclear translocation decreased the expression of SOX9 and CD44 under RFC, thereby attenuating the pluripotency of HepaRG-derived liver organoids.ConclusionsIn conclusion, we proposed a novel liver organoid construction method using rotating culture, by which the pluripotency of liver organoids so constructed is mediated by alpha 4 beta 6-integrin and YAP translocation. This work furthered the understanding in how the gravity vector orientation affects the construction of liver organoids and the related mechanotransductive pathways. |
| Keyword | Liver organoids HepaRG cells Rotating Flat Chamber Rotating culture YAP Mechanotransduction |
| DOI | 10.1186/s12915-024-02062-1 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:001355199600002 |
| WOS Research Area | Life Sciences & Biomedicine - Other Topics |
| WOS Subject | Biology |
| Funding Organization | National Key Research and Development Program of China {2021YFA0719302] ; National Natural Science Foundation of China {32130061, 32371376] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences {GJJSTU20220002] ; China Manned Space Flight Technology Project Chinese Space Station Experiment Project {YYWT0901EXP0701] |
| Classification | 二类/Q1 |
| Ranking | 1 |
| Contributor | Lue DY ; Long M |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/97160 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.【Zhong, Shaoyu & Zheng, Lu & Wu, Yi & Sun, Shujin & Lue, Dongyuan & Long, Mian】 Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing Key Lab Engn Construction & Mechanobiol, Beijing 100190, Peoples R China 2.【Zhong, Shaoyu & Zheng, Lu & Wu, Yi & Sun, Shujin & Lue, Dongyuan & Long, Mian】 Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China 3.【Zheng, Lu & Wu, Yi & Sun, Shujin & Lue, Dongyuan & Long, Mian】 Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 4.【Zhong, Shaoyu & Luo, Qing & Song, Guanbin】 Chongqing Univ, Coll Bioengn, Key Lab Biorheol Sci & Technol, Minist Educ, Chongqing 400044, Peoples R China |
| Recommended Citation GB/T 7714 | Zhong SY,Zheng L,Wu Y,et al. Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation[J]. BMC BIOLOGY,2024,22,1,:262.Rp_Au:Lue DY, Long M |
| APA | 钟绍宇.,郑璐.,武亿.,孙树津.,Luo, Qing.,...&龙勉.(2024).Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation.BMC BIOLOGY,22(1),262. |
| MLA | 钟绍宇,et al."Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation".BMC BIOLOGY 22.1(2024):262. |
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