| Neutrophils exhibit flexible migration strategies and trail formation mechanisms on varying adhesive substrates | |
Gao, Wenbo; Zhang XN(张晓宁) ; Hu WH(胡文慧); Han J(韩杰); Liu, Xiaoheng; Zhang Y(章燕); Long M(龙勉)
| |
| Source Publication | BIOMATERIALS
 |
| 2025-03 | |
| Volume | 314Pages:122881 |
| ISSN | 0142-9612 |
| Abstract | Substrate anchorage is essential for cell migration, and actin polymerization at cell front and myosin contractility at cell rear are known to govern cell forward movement. Yet their differential driving strategies for neutrophil migration on distinct adhesiveness substrates and their contributions to the migration-induced trail formation remain unclear. Here we explore the morphological changes, migration dynamics, and trail formation of neutrophils on ICAM-1 and PLL substrates, with a focus on the relationships among adhesive forces, traction forces, and out-of-plane forces. Results indicate that, on ICAM-1, neutrophil migration and trail formation rely on the coordinated interactions of Arp2/3 and myosin, along with biochemical regulation ( via Syk and calpain) of adhesion and de-adhesion. This pattern leads to traction forces being concentrated at relatively fewer adhesive sites, facilitating cell forward migration. On PLL, however, neutrophils primarily depend on Arp2/3-mediated actin polymerization, resulting in a broader distribution of traction forces and weaker adhesions, which allows for higher leading-edge migrating velocities. Elevated membrane tension and out-of-plane forces generated by bleb protrusions on PLL reduce the reliance on myosin-driven contraction at the trailing edge, enabling easier tail detachment through elastic recoil. This work highlights the differential impact of substrate adhesiveness on neutrophil migration and trail formation and dynamics, providing new insights into cell migration mechanisms and potential therapeutic targets for inflammatory and immune-related disorders. |
| Keyword | Neutrophil migration Trail release ICAM-1 PLL Migration strategy |
| DOI | 10.1016/j.biomaterials.2024.122881 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:001343979000001 |
| WOS Research Area | Engineering ; Materials Science |
| WOS Subject | Engineering, Biomedical ; Materials Science, Biomaterials |
| Funding Organization | National Natural Science Foundation of China {32250017, T2394514, 32130061, 12272389] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences {GJJSTU20220002] |
| Classification | 一类 |
| Ranking | 1 |
| Contributor | Zhang Y ; Long M ; Liu XH |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/97191 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.【Gao, Wenbo & Zhang, Xiaoning & Hu, Wenhui & Han, Jie & Zhang, Yan & Long, Mian】 Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China 2.【Gao, Wenbo & Zhang, Xiaoning & Hu, Wenhui & Han, Jie & Zhang, Yan & Long, Mian】 Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China 3.【Zhang, Xiaoning & Han, Jie & Zhang, Yan & Long, Mian】 Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 4.【Gao, Wenbo & Liu, Xiaoheng】 Sichuan Univ, Inst Biomed Engn, West China Sch Basic Med Sci & Forens Med, Chengdu 610041, Sichuan, Peoples R China |
| Recommended Citation GB/T 7714 | Gao, Wenbo,Zhang XN,Hu WH,et al. Neutrophils exhibit flexible migration strategies and trail formation mechanisms on varying adhesive substrates[J]. BIOMATERIALS,2025,314:122881.Rp_Au:Zhang Y, Long M, Liu XH |
| APA | Gao, Wenbo.,张晓宁.,胡文慧.,韩杰.,Liu, Xiaoheng.,...&龙勉.(2025).Neutrophils exhibit flexible migration strategies and trail formation mechanisms on varying adhesive substrates.BIOMATERIALS,314,122881. |
| MLA | Gao, Wenbo,et al."Neutrophils exhibit flexible migration strategies and trail formation mechanisms on varying adhesive substrates".BIOMATERIALS 314(2025):122881. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment