Knowledge Management System of Institue of Mechanics, CAS
| Micro-systems for Optical Protein-Chip | |
Jin G(靳刚) ; Wang ZH(王战会); Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China.
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| Source Publication | International Journal of Nonlinear Sciences and Numerical Simulation
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| 2002 | |
| Volume | 3Issue:3-4Pages:191-194 |
| ISSN | 1565-1339 |
| Abstract | Protein-Chip as micro-assays for the determination of protein interaction, the analysis, the identification and the purification of proteins has large potential applications. The Optical Protein-Chip is able to detect the multi-interaction of proteins and multi-bio-activities of molecules directly and simultaneously with no labeling. The chip is a small matrix on solid substrate containing multi-micro-area prepared by microfabrication with photolithography or soft lithography for surface patterning, and processed with surface modification which includes the physical, chemical, and bio-chemical modifications, etc. The ligand immobilization, such as protein immobilization, especially the oriented immobilization with low steric hindrance and high bio-specific binding activity between ligand and receptor is used to form a sensing surface. Each area of the pattern is corresponding to only one bioactivity. The interval between the areas is non-bioactive and optically extinctive. The affinity between proteins is used to realize non-labeling microassays for the determination of protein identification and protein interaction. The sampling of the chip is non-disturbing, performed with imaging ellipsometry and image processing on a database of proteins. |
| Subject Area | 力学 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000176903800007 |
| WOS Keyword | IMAGING ELLIPSOMETRY ; VISUALIZATION |
| WOS Research Area | Engineering ; Mathematics ; Mechanics ; Physics |
| WOS Subject | Engineering, Multidisciplinary ; Mathematics, Applied ; Mechanics ; Physics, Mathematical |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/17540 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China. |
| Recommended Citation GB/T 7714 | Jin G,Wang ZH,Jin, G . Micro-systems for Optical Protein-Chip[J]. International Journal of Nonlinear Sciences and Numerical Simulation,2002,3,3-4,:191-194. |
| APA | Jin G,Wang ZH,&Jin, G .(2002).Micro-systems for Optical Protein-Chip.International Journal of Nonlinear Sciences and Numerical Simulation,3(3-4),191-194. |
| MLA | Jin G,et al."Micro-systems for Optical Protein-Chip".International Journal of Nonlinear Sciences and Numerical Simulation 3.3-4(2002):191-194. |
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