| Micro-systems for Optical Protein-Chip |
| Jin G(靳刚) ; Wang ZH(王战会); Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China.
|
| Source Publication | International Journal of Nonlinear Sciences and Numerical Simulation
(IF:1.033[JCR-2018],1.106[5-Year]) |
| 2002
|
| Volume | 3Issue:3-4Pages:191-194 |
| ISSN | 1565-1339
|
| Abstract | Protein-Chip as micro-assays for the determination of protein interaction, the analysis, the identification and the purification of proteins has large potential applications. The Optical Protein-Chip is able to detect the multi-interaction of proteins and multi-bio-activities of molecules directly and simultaneously with no labeling. The chip is a small matrix on solid substrate containing multi-micro-area prepared by microfabrication with photolithography or soft lithography for surface patterning, and processed with surface modification which includes the physical, chemical, and bio-chemical modifications, etc. The ligand immobilization, such as protein immobilization, especially the oriented immobilization with low steric hindrance and high bio-specific binding activity between ligand and receptor is used to form a sensing surface. Each area of the pattern is corresponding to only one bioactivity. The interval between the areas is non-bioactive and optically extinctive. The affinity between proteins is used to realize non-labeling microassays for the determination of protein identification and protein interaction. The sampling of the chip is non-disturbing, performed with imaging ellipsometry and image processing on a database of proteins. |
| Subject Area | 力学
|
| Indexed By | SCI
|
| Language | 英语
|
| WOS ID | WOS:000176903800007
|
| WOS Keyword | IMAGING ELLIPSOMETRY
; VISUALIZATION
|
| WOS Research Area | Engineering
; Mathematics
; Mechanics
; Physics
|
| WOS Subject | Engineering, Multidisciplinary
; Mathematics, Applied
; Mechanics
; Physics, Mathematical
|
| Citation statistics |
|
| Document Type | 期刊论文
|
| Identifier | http://dspace.imech.ac.cn/handle/311007/17540
|
| Collection | 力学所知识产出(1956-2008)
|
| Corresponding Author | Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China. |
Recommended Citation
GB/T 7714 |
Jin G,Wang ZH,Jin, G . Micro-systems for Optical Protein-Chip[J]. International Journal of Nonlinear Sciences and Numerical Simulation,2002,3,3-4,:191-194.
|
| APA |
Jin G,Wang ZH,&Jin, G .(2002).Micro-systems for Optical Protein-Chip.International Journal of Nonlinear Sciences and Numerical Simulation,3(3-4),191-194.
|
| MLA |
Jin G,et al."Micro-systems for Optical Protein-Chip".International Journal of Nonlinear Sciences and Numerical Simulation 3.3-4(2002):191-194.
|
Edit Comment