| Microfluidic effects of transporting signaling components in cell coculture chips | |
Hu GQ(胡国庆) ; Li DQ; Dongqing Li
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| Source Publication | Microfluidics and Nanofluidics
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| 2009 | |
| Volume | 6Issue:1Pages:99-107 |
| ISSN | 1613-4982 |
| Abstract | A theoretical model has been developed to investigate the microfluidic transport of the signaling chemicals in the cell coculture chips. Using an epidermal growth factor (EGF)-like growth factor as the sample chemical, the effects of velocities and channel geometry were studied for the continuous-flow microchannel bioreactors. It is found that different perfusion velocities must be applied in the parallel channels to facilitate the communication, i.e., transport of the signaling component, between the coculture channels. Such communication occurs in a unidirectional way because the signaling chemicals can only flow from the high velocity area to the low velocity area. Moreover, the effect of the transport of the signaling component between the coculture channels on the growth of the monolayer cells and the multicellular tumor spheroid (MTS) in the continuous-flow coculture environment were simulated using 3D models. The numerical results demonstrated that the concentration gradients will induce the heterogeneous growth of the cells and the MTSs, which should be taken into account in designing the continuous-flow perfusion bioreactor for the cell coculture research. |
| Keyword | Microfluidics Cell Coculture Numerical Simulation Concentration Gradients Culture Bioreactor Growth Microchannel Design System Differentiation Proliferation Hepatocytes Receptors |
| Subject Area | 微机电系统中的流体力学问题 |
| DOI | 10.1007/s10404-008-0304-6 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000262283400010 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/28782 |
| Collection | 非线性力学国家重点实验室 |
| Corresponding Author | Dongqing Li |
| Recommended Citation GB/T 7714 | Hu GQ,Li DQ,Dongqing Li. Microfluidic effects of transporting signaling components in cell coculture chips[J]. Microfluidics and Nanofluidics,2009,6,1,:99-107. |
| APA | Hu GQ,Li DQ,&Dongqing Li.(2009).Microfluidic effects of transporting signaling components in cell coculture chips.Microfluidics and Nanofluidics,6(1),99-107. |
| MLA | Hu GQ,et al."Microfluidic effects of transporting signaling components in cell coculture chips".Microfluidics and Nanofluidics 6.1(2009):99-107. |
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| File Name/Size | DocType | Version | Access | License | ||
| 3.pdf（560KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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