| Detection of hepatitis B virus markers using a biosensor based on imaging ellipsometry | |
Qi C(齐财); Zhu W; Niu Y ; Zhang HG; Zhu GY; Meng YH(孟永宏); Chen S(陈涉); Jin G(靳刚); Jin G
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| Source Publication | Journal of Viral Hepatitis
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| 2009 | |
| Volume | 16Issue:11Pages:822-832 |
| ISSN | 1352-0504 |
| Abstract | A biosensor based on imaging ellipsometry (BIE) has been developed and validated in 169 patients for detecting five markers of hepatitis B virus (HBV) infection. The methodology has been established to pave the way for clinical diagnosis, including ligand screening, determination of the sensitivity, set-up of cut-off values (CoVs) and comparison with other clinical methods. A matrix assay method was established for ligand screening. The CoVs of HBV markers were derived with the help of receiver operating characteristic curves. Enzyme-linked immunosorbent assay (ELISA) was the reference method. Ligands with high bioactivity were selected and sensitivities of 1 ng/mL and 1 IU/mL for hepatitis B surface antigen (HBsAg) and surface antibody (anti-HBs) were obtained respectively. The CoVs of HBsAg, anti-HBs, hepatitis B e antigen, hepatitis B e antibody and core antibody were as follows: 15%, 18%, 15%, 20% and 15%, respectively, which were the percentages over the values of corresponding ligand controls. BIE can simultaneously detect up to five markers within 1 h with results in acceptable agreement with ELISA, and thus shows a potential for diagnosing hepatitis B with high throughput. |
| Keyword | Hepatitis b Imaging Ellipsometry Label-free Biosensor Microfluidic Surface-antigen Assay Immunosensor Hbsag Gene Chip Hbc |
| DOI | 10.1111/j.1365-2893.2009.01123.x |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000270729700009 |
| WOS Keyword | SURFACE-ANTIGEN ; ASSAY ; IMMUNOSENSOR ; HBSAG ; GENE ; CHIP ; HBC |
| WOS Research Area | Gastroenterology & Hepatology ; Infectious Diseases ; Virology |
| WOS Subject | Gastroenterology & Hepatology ; Infectious Diseases ; Virology |
| Funding Organization | Chinese Academy of Sciences [KJCX2-YW-M04, -M03] ; National Basic Research Program of China [2009CB320302] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/33559 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Jin G |
| Recommended Citation GB/T 7714 | Qi C,Zhu W,Niu Y,et al. Detection of hepatitis B virus markers using a biosensor based on imaging ellipsometry[J]. Journal of Viral Hepatitis,2009,16,11,:822-832. |
| APA | 齐财.,Zhu W.,Niu Y.,Zhang HG.,Zhu GY.,...&Jin G.(2009).Detection of hepatitis B virus markers using a biosensor based on imaging ellipsometry.Journal of Viral Hepatitis,16(11),822-832. |
| MLA | 齐财,et al."Detection of hepatitis B virus markers using a biosensor based on imaging ellipsometry".Journal of Viral Hepatitis 16.11(2009):822-832. |
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