Effects of forced solution flow on lysozyme crystal growth

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	Effects of forced solution flow on lysozyme crystal growth
	Yu Y(于泳); Liu YH(刘玉红); Wang X(王翾); Oberthür D; Dierks K; Betzel C; Yong Yu(于泳）
发表期刊	Crystal Research and Technology
	2010
卷号	45 期号:4 页码:380-386
ISSN	0232-1300
摘要	In order to study quantitatively the effects of forced solution on crystal growth, we designed a new set of experimental equipment, in particular, a microchannel mixer was used as crystallization container so that the consumption of protein samples was much reduced and thus an exact syringe pump could be used for precise control of the flow rates. Since the mixer’s section was designed to be rectangular, the solution velocity in its center was steady and constant, and thus repeatable experiments were facilitated. Experimental results showed that the effects of forced solution on protein crystal growth were different under different levels of supersaturation, and new results were obtained for cases of high supersaturation. When the supersaturation is σ = 2.3, with increasing flow rates the growth rates of the lysozyme crystal’s (110) face hardly change when the flow rates are lower than 1300 μm/s, and decrease quickly afterwards. When the flow rate reaches 2000 μm/s, the crystal nearly ceases to grow. When the supersaturation is σ = 2.7, with increasing flow rates the (110) face growth rates increase at the beginning then reach the maximum values at 1700 μm/s – 1900 μm/s and decrease afterwards, approaching zero or so when the flow rate reaches 12000 μm/s. The higher the supersaturation, the larger the flow rate at which the crystal ceases to grow. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
关键词	Protein Crystallization Fluid Flow Impurities Mass Transfer Lysozyme.
学科领域	交叉与边缘领域的力学
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收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000276599200007
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/43244
专题	微重力重点实验室
通讯作者	Yong Yu(于泳）
推荐引用方式 GB/T 7714	Yu Y,Liu YH,Wang X,et al. Effects of forced solution flow on lysozyme crystal growth[J]. Crystal Research and Technology,2010,45,4,:380-386.
APA	Yu Y.,Liu YH.,Wang X.,Oberthür D.,Dierks K.,...&Yong Yu.(2010).Effects of forced solution flow on lysozyme crystal growth.Crystal Research and Technology,45(4),380-386.
MLA	Yu Y,et al."Effects of forced solution flow on lysozyme crystal growth".Crystal Research and Technology 45.4(2010):380-386.

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