| Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching | |
Zhong Y; Kong D(孔冬) ; Dai LH(戴兰宏); Ji BH(季葆华); Ji, BH (reprint author), Beijing Inst Technol, Biomech & Biomat Lab, Dept Appl Mech, Beijing 100081, Peoples R China
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| Source Publication | Cellular and Molecular Bioengineering
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| 2011 | |
| Volume | 4Issue:3Pages:442-456 |
| ISSN | 1865-5025 |
| Abstract | The adhesion-based cell mechanosensitivity plays central roles in many physiological and pathological processes. Recently, quantitative understanding of cell responses to external force has been intensively pursued. However, the frequency dependent cell responses to the substrate stretching have not yet been fully understood. Here we developed a multiscale modeling framework for studying cell reorientation behaviors under substrate stretching, in which the mechano-chemical coupling at molecular, subcellular, and cellular scales was considered. The effect of matrix stiffness was also considered in a FEM based mechano-chemical coupling simulation. We showed that the collapsing time of focal adhesion decreases with the increasing of the loading frequency, however, the cell reorientation time exhibits a biphasic frequency-dependent behavior. Our results suggested that this biphasic behavior might be caused by the competition between the frequency-dependent collapsing of focal adhesions and the less frequency-dependent formation of stress fibers aligning away from the loading direction. At the low loading frequency, the collapsing of focal adhesion dominates the reorientation process, however, at the high loading frequency the polymerization of stress fiber dominates the reorientation. Moreover, we showed that the compliance of matrix may help accelerate the cell reorientation because focal adhesion is prone to be instable on soft matrix. |
| Keyword | Cell Adhesion Mechanosensitivity Stress Fiber Mechano-chemical Coupling Multiscale Modeling Loading Frequency Smooth-muscle-cells Stress Fibers Orientation Response Mechanical Force Kinematic Model Catch Bonds Dynamics Actin Integrin Mechanosensitivity |
| Subject Area | Cell Biology ; Biophysics |
| DOI | 10.1007/s12195-011-0187-6 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000297866300012 |
| WOS Keyword | SMOOTH-MUSCLE-CELLS ; STRESS FIBERS ; ORIENTATION RESPONSE ; MECHANICAL FORCE ; KINEMATIC MODEL ; CATCH BONDS ; DYNAMICS ; ACTIN ; INTEGRIN ; MECHANOSENSITIVITY |
| WOS Research Area | Cell Biology ; Biophysics |
| WOS Subject | Cell & Tissue Engineering ; Biophysics ; Cell Biology |
| Funding Organization | This research was supported by the National Natural Science Foundation of China through Grant No. 10732050, 10872115 and 11025208. LD acknowledges the support from the key project of Chinese Academy of Sciences through KJCX2-YW-M04 and KJCX-SW-L08. |
| Department | LNM冲击动力学与新型材料力学性能 |
| Classification | Q4 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/44908 |
| Collection | 非线性力学国家重点实验室 |
| Corresponding Author | Ji, BH (reprint author), Beijing Inst Technol, Biomech & Biomat Lab, Dept Appl Mech, Beijing 100081, Peoples R China |
| Recommended Citation GB/T 7714 | Zhong Y,Kong D,Dai LH,et al. Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching[J]. Cellular and Molecular Bioengineering,2011,4,3,:442-456. |
| APA | Zhong Y,孔冬,戴兰宏,季葆华,&Ji, BH .(2011).Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching.Cellular and Molecular Bioengineering,4(3),442-456. |
| MLA | Zhong Y,et al."Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching".Cellular and Molecular Bioengineering 4.3(2011):442-456. |
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