| Investigation of the interaction between a bivalent aptamer and thrombin by AFM | |
Ge L; Jin G(靳刚) ; Fang XH; Jin, G; Chinese Acad Sci, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China.
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| Source Publication | LANGMUIR
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| 2012 | |
| Volume | 28Issue:1Pages:707-713 |
| ISSN | 0743-7463 |
| Abstract | Aptamers are a new class of molecular probes for protein recognition, detection, and inhibition. Multivalent aptamer protein binding through aptamer assembly has been currently developed as an effective way to achieve higher protein affinity and selectivity. In this study, the specific interaction between bivalent aptamer Bi-8S and thrombin has been measured directly and quantitatively by atomic force microscopy to investigate the unbinding dynamics and dissociation energy landscape of the multivalent interaction. Bivalent aptamer Bi-8S contains thrombin's two aptamers, 15apt and 27apt, which are linked by eight spacer phosphoramidites. The results revealed the sequential dissociation of the two aptamers. Moreover, the dynamic force spectroscopy data revealed that the 27apt's binding to the thrombin remains largely unaffected by the eight-spacer phosphoramidites within Bi-8S. In contrast, the eight-spacer phosphoramidites stabilized the 15apt-thrombin binding. |
| Keyword | Dynamic Force Spectroscopy Single-molecule Energy Landscape Sinorhizobium-meliloti Membrane-proteins Receptor-binding Dna-molecules Rna Ligands Inhibitor Recognition |
| Subject Area | 生物力学 |
| URL | 查看原文 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000298904900089 |
| Funding Organization | We thank Le Mu for experimental advice and Mehdi Damaghi and Prof. Daniel Muller for helpful discussions. This work was supported by the Chinese Academy of Sciences and the National Basic Research Program of China (2011CB911001) and the NSFC (no. 20821003). |
| Department | NML纳米生物光学 |
| Classification | 一类 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/46555 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Jin, G; Chinese Acad Sci, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China. |
| Recommended Citation GB/T 7714 | Ge L,Jin G,Fang XH,et al. Investigation of the interaction between a bivalent aptamer and thrombin by AFM[J]. LANGMUIR,2012,28,1,:707-713. |
| APA | Ge L,靳刚,Fang XH,Jin, G,&Chinese Acad Sci, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China..(2012).Investigation of the interaction between a bivalent aptamer and thrombin by AFM.LANGMUIR,28(1),707-713. |
| MLA | Ge L,et al."Investigation of the interaction between a bivalent aptamer and thrombin by AFM".LANGMUIR 28.1(2012):707-713. |
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