Effects of Mechanical Stretching on the Morphology and Cytoskeleton of Vaginal Fibroblasts from Women with Pelvic Organ Prolapse

doi:10.3390/ijms16059406

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	Effects of Mechanical Stretching on the Morphology and Cytoskeleton of Vaginal Fibroblasts from Women with Pelvic Organ Prolapse
	Wang SM; Zhang ZY; Lv DY(吕东媛); Xu QX; Zhang, ZY (reprint author), Capital Med Univ, Beijing Chaoyang Hosp, Dept Obstet & Gynecol, Beijing 100020, Peoples R China.
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2015-05
卷号	16 期号:5 页码:9406-9419
ISSN	1422-0067
摘要	Mechanical load and postmenopausal hypoestrogen are risk factors for pelvic organ prolapse (POP). In this study, we applied a 0.1-Hz uniaxial cyclic mechanical stretching (CS) with 10% elongation and 10(-8) M 17--estradiol to vaginal fibroblasts isolated from postmenopausal women with or without POP to investigate the effects of CS and estrogen on cell morphology and cytoskeletons of normal and POP fibroblasts. Under static culture condition, POP fibroblasts exhibited lower cell circularity and higher relative fluorescence intensities (RFIs) of F-actin, -tubulin and vimentin. When cultured with CS, all fibroblasts grew perpendicular to the force and exhibited a decreased cell projection area, cell circularity and increased cell length/width ratio; normal fibroblasts exhibited increased RFIs of all three types of cytoskeleton, and POP fibroblasts exhibited a decreased RFI of F-actin and no significant differences of -tubulin and vimentin. After being cultured with 17--estradiol and CS, normal fibroblasts no longer exhibited significant changes in the cell projection area and the RFIs of F-actin and -tubulin; POP fibroblasts exhibited no significant changes in cell circularity, length/width ratio and F-actin even with the increased RFIs of -tubulin and vimentin. These findings suggest that POP fibroblasts have greater sensitivity to and lower tolerance for mechanical stretching, and estrogen can improve the prognosis.
关键词	Cyclical Mechanical Stretch Estrogen Pelvic Organ Prolapse Vaginal Fibroblasts
DOI	10.3390/ijms16059406
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000356241400019
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
项目资助者	National Key Technology Research and Development Program during the 11th Five-Year Plan Period [2007BAI04B05]
课题组名称	NML分子-细胞生物力学与空间生命科学
论文分区	二类/Q2
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/54970
专题	微重力重点实验室
通讯作者	Zhang, ZY (reprint author), Capital Med Univ, Beijing Chaoyang Hosp, Dept Obstet & Gynecol, Beijing 100020, Peoples R China.
推荐引用方式 GB/T 7714	Wang SM,Zhang ZY,Lv DY,et al. Effects of Mechanical Stretching on the Morphology and Cytoskeleton of Vaginal Fibroblasts from Women with Pelvic Organ Prolapse[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2015,16,5,:9406-9419.
APA	Wang SM,Zhang ZY,吕东媛,Xu QX,&Zhang, ZY .(2015).Effects of Mechanical Stretching on the Morphology and Cytoskeleton of Vaginal Fibroblasts from Women with Pelvic Organ Prolapse.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,16(5),9406-9419.
MLA	Wang SM,et al."Effects of Mechanical Stretching on the Morphology and Cytoskeleton of Vaginal Fibroblasts from Women with Pelvic Organ Prolapse".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 16.5(2015):9406-9419.

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