| Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer | |
Huang CH; Li JX ; Tang Y; Wang CX; Hou CJ; Huo DQ; Chen YY(陈艳艳); Jin G(靳刚); Jin, G (reprint author), CAS, Inst Mech, Beijing 100190, Peoples R China.
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| Source Publication | MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
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| 2011 | |
| Volume | 31Issue:7Pages:1609-1613 |
| ISSN | 0928-4931 |
| Abstract | In this study, a practicable method for the detection of Riemerella anatipestifer (R. anatipestifer) using biosensor based on imaging ellipsometry (BIE) is described. The method is performed by immobilizing anti-R. anatipestifer egg yolk immunoglubilin (IgY) onto modified chemistry surface to form sensing layer. Antigen captured by sensing layer can then be quantitatively measured through imaging ellipsometry in grayscale format. The results demonstrate that it can detect R. anatipestifer as low as 5.2 x 10(3) CFU/mL. Furthermore, the proposed method can realize specific discrimination for multiple serotypes of 1,2, 4, and 14 of R. anatipestifer. It has the advantages of rapid, simple, high sensitivity and low cost. (C) 2011 Elsevier B.V. All rights reserved. |
| Keyword | Biosensor Imaging Ellipsometry Riemerella Anatipestifer Detection |
| DOI | 10.1016/j.msec.2011.05.018 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000295953900051 |
| WOS Keyword | PASTEURELLA-ANATIPESTIFER ; ESCHERICHIA-COLI ; IMMUNOSENSOR ; DUCKS ; VISUALIZATION ; INFECTION |
| WOS Research Area | Materials Science |
| WOS Subject | Materials Science, Biomaterials |
| Funding Organization | Natural Science Foundation [20845003, 10804083] ; Ministry of Science and Technology of China [2008AA02Z419] ; Chinese Academy of Sciences [KJCX2.YW.M04] ; Natural Science Foundation of Chongqing [CSTC2008 BB1245] ; Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences |
| Department | NML纳米生物光学 |
| Classification | 一类 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/58582 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Jin, G (reprint author), CAS, Inst Mech, Beijing 100190, Peoples R China. |
| Recommended Citation GB/T 7714 | Huang CH,Li JX,Tang Y,et al. Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2011,31,7,:1609-1613. |
| APA | Huang CH.,Li JX.,Tang Y.,Wang CX.,Hou CJ.,...&Jin, G .(2011).Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,31(7),1609-1613. |
| MLA | Huang CH,et al."Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 31.7(2011):1609-1613. |
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| JourArSuppl2016-014.（668KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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