| Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling | |
Li N(李宁) ; Yang H(杨浩); Wang ML; Lv SQ(吕守芹); Zhang Y(章燕); Long M(龙勉)
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| Source Publication | MOLECULAR BIOLOGY OF THE CELL
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| 2018-02-15 | |
| Volume | 29Issue:4Pages:408-418 |
| ISSN | 1059-1524 |
| Abstract | Lymphocyte function-associated antigen-1 (LFA-1) and macrophage-1 antigen (Mac-1) and their counterreceptors such as intercellular cell adhesion molecules (ICAM-1 and ICAM-2), junctional adhesion molecules (JAM-A, JAM-C), and receptors for advanced glycation end products (RAGE) are crucial for promoting polymorphonuclear leukocyte (neutrophil, PMN) recruitment. The underlying mechanisms of ligand-specific bindings in this cascade remain incompletely known. We compared the dynamic force spectra for various LFA-1/Mac1-ligand bonds using single-molecule atomic force microscopy (AFM) and tested their functions in mediating PMN recruitment under in vitro shear flow. Distinct features of bond rupture forces and lifetimes were uncovered for these ligands, implying their diverse roles in regulating PMN adhesion on endothelium. LFA-1 dominates PMN adhesion on ICAM-1 and ICAM-2, while Mac-1 mediates PMN adhesion on RAGE, JAM-A, and JAM-C, which is consistent with their bond strength. All ligands can trigger PMN spreading and polarization, in which Mac-1 seems to induce outside-in signaling more effectively. LFA-1-ICAM-1 and LFA-1/Mac-1-JAM-C bonds can accelerate PMN crawling under high shear stress, presumably due to their high mechanical strength. This work provides new insight into basic molecular mechanisms of physiological ligands of beta 2 integrins in PMN recruitment. |
| DOI | 10.1091/mbc.E16-12-0827 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000425859100005 |
| WOS Keyword | INFLAMMATORY CELL RECRUITMENT ; HIGH-STRENGTH STATES ; BETA(2) INTEGRIN ; ENDOTHELIAL-CELLS ; SHEAR-FLOW ; IN-VIVO ; TRANSENDOTHELIAL MIGRATION ; CONFORMATIONAL-CHANGES ; LEUKOCYTE RECRUITMENT ; MOLECULE-1 ICAM-1 |
| WOS Research Area | Cell Biology |
| WOS Subject | Cell Biology |
| Funding Organization | National Natural Science Foundation of China [31230027, 31110103918, 31300776] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences [XDA01030102, XDB22040101, QYZDJ-SSW-JSC018] ; National Key Research and Development Program of China [2016YFA0501601] ; Visiting Scholar Foundation of the Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education [CQKLBST-2015-002] |
| Classification | 二类 |
| Ranking | 1 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/77801 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Ctr Biomech & Bioengn, Natl Micrograv Lab, Key Lab Micrograv, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 4.Chongqing Univ, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400044, Peoples R China |
| Recommended Citation GB/T 7714 | Li N,Yang H,Wang ML,et al. Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling[J]. MOLECULAR BIOLOGY OF THE CELL,2018,29,4,:408-418. |
| APA | 李宁,杨浩,Wang ML,吕守芹,章燕,&龙勉.(2018).Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling.MOLECULAR BIOLOGY OF THE CELL,29(4),408-418. |
| MLA | 李宁,et al."Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling".MOLECULAR BIOLOGY OF THE CELL 29.4(2018):408-418. |
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| IrJ2018186.pdf（1832KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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