| Label-Free Immunoassay of Carcinoembryonic Antigen by Microfluidic Channel Biosensor Based on Imaging Ellipsometry and Its Clinic Application | |
Huang CH1; Wang BX2; Chen YY(陈艳艳)3 ; Jin G(靳刚)4
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| Source Publication | INTEGRATED FERROELECTRICS
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| 2020-07-23 | |
| Volume | 209Issue:1Pages:19-29 |
| ISSN | 1058-4587 |
| Abstract | This study represents a label-free immunoassay for carcinoembryonic antigen (CEA) by microfluidic biosensor based on imaging ellipsometry (BIE) and its application is further validated by clinic sera. The sandwich format of analysis configuration was adopted, in which Rat-mAb was served as capture antibody, rabbit anti-CEA polyclonal antibody (rabbit-pAb) as detection antibody and goat anti-rabbit IgG (goat-IgG) as an enhancer added to increase detection signal. The methodology includes rat-mAb concentration screening, rabbit-pAb and goat-IgG concentration optimization, creation of dose-response curve and determination of cutoff value. Results show the linear range is from 4.0-64.0 ng/ml with a lower limit of 1.0 ng/ml (S/N = 3). Application of the BIE to clinic samples demonstrated that the relation coefficient is 0.94 and substantial agreement with the results of ECLIA's (Kappa = 0.733,U = 4.35 > U-0.01). The proposed method exhibits the potential for application. |
| Keyword | Imaging ellipsometry carcinoembryonic antigen immunoassay |
| DOI | 10.1080/10584587.2020.1728800 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000547668500003 |
| WOS Keyword | FLOW-INJECTION IMMUNOASSAY ; OFF-NULL ELLIPSOMETRY ; IMMUNOSENSOR ; VISUALIZATION ; VALIDATION ; LAYERS ; ASSAY |
| WOS Research Area | Engineering ; Physics |
| WOS Subject | Engineering, Electrical & Electronic ; Physics, Applied ; Physics, Condensed Matter |
| Funding Organization | Open Fund of Chongqing Key Laboratory of Industrial Fermentation Microorganism[LIFM201712] ; NSF of Chongqing[cstc2016shmszx80080] ; NSF of Chongqing[cstc2019jscx-msxm1542] ; Chongqing Education Board[KJ1605701] |
| Classification | Q4 |
| Ranking | 4 |
| Contributor | Huang, Chenghong |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/84744 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Chongqing Univ Sci & Technol, Chongqing Key Lab Ind Fermentat Microorganism, Shapingba 401331, Peoples R China; 2.Chongqing Light Ind Polytech Coll, Jiulongpo, Peoples R China; 3.Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Beijing, Peoples R China; 4.Chinese Acad Sci, Inst Mech, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Huang CH,Wang BX,Chen YY,et al. Label-Free Immunoassay of Carcinoembryonic Antigen by Microfluidic Channel Biosensor Based on Imaging Ellipsometry and Its Clinic Application[J]. INTEGRATED FERROELECTRICS,2020,209,1,:19-29.Rp_Au:Huang, Chenghong |
| APA | Huang CH,Wang BX,陈艳艳,&靳刚.(2020).Label-Free Immunoassay of Carcinoembryonic Antigen by Microfluidic Channel Biosensor Based on Imaging Ellipsometry and Its Clinic Application.INTEGRATED FERROELECTRICS,209(1),19-29. |
| MLA | Huang CH,et al."Label-Free Immunoassay of Carcinoembryonic Antigen by Microfluidic Channel Biosensor Based on Imaging Ellipsometry and Its Clinic Application".INTEGRATED FERROELECTRICS 209.1(2020):19-29. |
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| Jp2020188.pdf（1447KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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