| Mapping the morphological identifiers of distinct conformations via the protein translocation current in nanopores | |
Zhang MK(张明坤)1,2,3,4,5; Chen SB(陈深宝)1,2,3 ; Hu JR(胡锦荣)1,2,3; Ding QH(丁奇寒)1,2,3; Li LD(李林达)1,2,3; Lv SQ(吕守芹)1,2,3; Long M(龙勉)1,2,3
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| Corresponding Author | Lu, Shouqin([email protected]) ; Long, Mian([email protected]) |
| Source Publication | NANOSCALE
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| 2021-03-28 | |
| Volume | 13Issue:12Pages:14 |
| ISSN | 2040-3364 |
| Abstract | Conformational changes of proteins play a vital role in implementing their functions and revealing the underlying mechanisms in various biological processes. It is still challenging to monitor protein conformations with temporal fingerprints of current-resistance pulses in the nanopore technique. Here the low-resolution morphologies of different conformations of a typical integrin, alpha(x)beta(2), were estimated via relative blockade currents simulated from all-atom molecular dynamics (MD). Distinct conformational states of alpha(x)beta(2) were directly explained by the volume and shape identifiers. Protein modulation in ionic current was analyzed from the conductivity distribution inside the protein-blocked nanopore. Combining a discrete model with spheroidal approximation, a MD-based approach was developed to theoretically predict the volume and shape of the nanopore for sensing alpha(x)beta(2). This method was also applicable in specifying morphological identifiers of six other proteins, and the theoretical predictions are in good agreement with the experimental measurements. These results potentiated the validity of this method for the conformational identification of proteins in nanopores. |
| DOI | 10.1039/d0nr07413f |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000635775000012 |
| WOS Research Area | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| WOS Subject | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied |
| Classification | 一类 |
| Ranking | 1 |
| Contributor | Lu, Shouqin ; Long, Mian |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/86444 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab,Beijing Key Lab Engn Construct, Beijing 100190, Peoples R China; 2.Chinese Acad Sci, Inst Mech, CAS Ctr Excellence Complex Syst Mech, Beijing 100190, Peoples R China; 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China; 4.Chinese Acad Sci, Chongqing Engn Res Ctr High Resolut & Three Dimen, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China; 5.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang MK,Chen SB,Hu JR,et al. Mapping the morphological identifiers of distinct conformations via the protein translocation current in nanopores[J]. NANOSCALE,2021,13,12,:14.Rp_Au:Lu, Shouqin, Long, Mian |
| APA | 张明坤.,陈深宝.,胡锦荣.,丁奇寒.,李林达.,...&龙勉.(2021).Mapping the morphological identifiers of distinct conformations via the protein translocation current in nanopores.NANOSCALE,13(12),14. |
| MLA | 张明坤,et al."Mapping the morphological identifiers of distinct conformations via the protein translocation current in nanopores".NANOSCALE 13.12(2021):14. |
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| Jp2021F178.pdf（6632KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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