E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity

doi:10.1096/fj.202000662RR

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	E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity
	Huang DD(黄丹丹)1,2,3; Ding QH(丁奇寒)1,2,3; Chen SB(陈深宝)1,2,3 ; Lv SQ(吕守芹)1,2,3 ; Zhang Y(章燕)1,2,3 ; Long M(龙勉)1,2,3
通讯作者	Zhang, Yan([email protected]) ; Long, Mian([email protected])
发表期刊	FASEB JOURNAL
	2021-05-01
卷号	35 期号:5 页码:15
ISSN	0892-6638
摘要	Transendothelial migration (TEM) of neutrophils under blood flow is critical in the inflammatory cascade. However, the role of endothelial plasticity in this process is not fully understood. Therefore, we used an in vitro model to test the dynamics of human polymorphonuclear neutrophil (PMN) TEM across lipopolysaccharide-treated human umbilical vein endothelial cell (HUVEC) monolayers. Interestingly, shRNA-E-selectin knockdown in HUVECs destabilized endothelial junctional integrity by reducing actin branching and increasing stress fiber at cell-cell junctions. This process is accomplished by downregulating the activation of cortactin and Arp2/3, which in turn alters the adhesive function of VE-cadherin, enhancing PMN transmigration. Meanwhile, redundant P-selectins possess overlapping functions in E-selectin-mediated neutrophil adhesion, and transmigration. These results demonstrate, to our knowledge, for the first time, that E-selectins negatively regulate neutrophil transmigration through alterations in endothelial plasticity. Furthermore, it improves our understanding of the mechanisms underlying actin remodeling, and junctional integrity, in endothelial cells mediating leukocyte TEM.
关键词	cytoskeletal remodeling endothelial integrity E-selectin neutrophil transmigration
DOI	10.1096/fj.202000662RR
收录类别	SCI
语种	英语
WOS记录号	WOS:000645260100005
关键词[WOS]	ARP2/3 COMPLEX ; CELL-ADHESION ; LEUKOCYTE ADHESION ; ACTIN ; DYNAMICS ; LAMELLIPODIA ; CORTACTIN ; MOLECULES ; MIGRATION ; PROTEINS
WOS研究方向	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biology ; Cell Biology
资助项目	National Natural Science Foundation of China (NSFC)[31627804] ; National Natural Science Foundation of China (NSFC)[91642203] ; National Natural Science Foundation of China (NSFC)[11772345] ; National Natural Science Foundation of China (NSFC)[91539119] ; National Key Rsearch and Development Program of China[2016YFA0501601] ; Chinese Academy of Sciences Strategic Priority Research Program[XDB22040101] ; Frontier Science Key Project[QYZDJ--SSW--JSC018]
项目资助者	National Natural Science Foundation of China (NSFC) ; National Key Rsearch and Development Program of China ; Chinese Academy of Sciences Strategic Priority Research Program ; Frontier Science Key Project
论文分区	二类/Q1
力学所作者排名	1
RpAuthor	Zhang, Yan ; Long, Mian
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/86896
专题	微重力重点实验室
作者单位	1.Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Ctr Biomech & Bioengn,Key Lab Micrograv, Beijing 100190, Peoples R China; 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China; 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Huang DD,Ding QH,Chen SB,et al. E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity[J]. FASEB JOURNAL,2021,35,5,:15.Rp_Au:Zhang, Yan, Long, Mian
APA	黄丹丹,丁奇寒,陈深宝,吕守芹,章燕,&龙勉.(2021).E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity.FASEB JOURNAL,35(5),15.
MLA	黄丹丹,et al."E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity".FASEB JOURNAL 35.5(2021):15.

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