| E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity | |
Huang DD(黄丹丹)1,2,3; Ding QH(丁奇寒)1,2,3; Chen SB(陈深宝)1,2,3 ; Lv SQ(吕守芹)1,2,3; Zhang Y(章燕)1,2,3; Long M(龙勉)1,2,3
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| Corresponding Author | Zhang, Yan([email protected]) ; Long, Mian([email protected]) |
| Source Publication | FASEB JOURNAL
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| 2021-05-01 | |
| Volume | 35Issue:5Pages:15 |
| ISSN | 0892-6638 |
| Abstract | Transendothelial migration (TEM) of neutrophils under blood flow is critical in the inflammatory cascade. However, the role of endothelial plasticity in this process is not fully understood. Therefore, we used an in vitro model to test the dynamics of human polymorphonuclear neutrophil (PMN) TEM across lipopolysaccharide-treated human umbilical vein endothelial cell (HUVEC) monolayers. Interestingly, shRNA-E-selectin knockdown in HUVECs destabilized endothelial junctional integrity by reducing actin branching and increasing stress fiber at cell-cell junctions. This process is accomplished by downregulating the activation of cortactin and Arp2/3, which in turn alters the adhesive function of VE-cadherin, enhancing PMN transmigration. Meanwhile, redundant P-selectins possess overlapping functions in E-selectin-mediated neutrophil adhesion, and transmigration. These results demonstrate, to our knowledge, for the first time, that E-selectins negatively regulate neutrophil transmigration through alterations in endothelial plasticity. Furthermore, it improves our understanding of the mechanisms underlying actin remodeling, and junctional integrity, in endothelial cells mediating leukocyte TEM. |
| Keyword | cytoskeletal remodeling endothelial integrity E-selectin neutrophil transmigration |
| DOI | 10.1096/fj.202000662RR |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000645260100005 |
| WOS Keyword | ARP2/3 COMPLEX ; CELL-ADHESION ; LEUKOCYTE ADHESION ; ACTIN ; DYNAMICS ; LAMELLIPODIA ; CORTACTIN ; MOLECULES ; MIGRATION ; PROTEINS |
| WOS Research Area | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology |
| WOS Subject | Biochemistry & Molecular Biology ; Biology ; Cell Biology |
| Funding Project | National Natural Science Foundation of China (NSFC)[31627804] ; National Natural Science Foundation of China (NSFC)[91642203] ; National Natural Science Foundation of China (NSFC)[11772345] ; National Natural Science Foundation of China (NSFC)[91539119] ; National Key Rsearch and Development Program of China[2016YFA0501601] ; Chinese Academy of Sciences Strategic Priority Research Program[XDB22040101] ; Frontier Science Key Project[QYZDJ--SSW--JSC018] |
| Funding Organization | National Natural Science Foundation of China (NSFC) ; National Key Rsearch and Development Program of China ; Chinese Academy of Sciences Strategic Priority Research Program ; Frontier Science Key Project |
| Classification | 二类/Q1 |
| Ranking | 1 |
| Contributor | Zhang, Yan ; Long, Mian |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/86896 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Ctr Biomech & Bioengn,Key Lab Micrograv, Beijing 100190, Peoples R China; 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China; 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Huang DD,Ding QH,Chen SB,et al. E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity[J]. FASEB JOURNAL,2021,35,5,:15.Rp_Au:Zhang, Yan, Long, Mian |
| APA | 黄丹丹,丁奇寒,陈深宝,吕守芹,章燕,&龙勉.(2021).E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity.FASEB JOURNAL,35(5),15. |
| MLA | 黄丹丹,et al."E-selectin negatively regulates polymorphonuclear neutrophil transmigration through altered endothelial junction integrity".FASEB JOURNAL 35.5(2021):15. |
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| 662RR.pdf（2008KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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