| Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry | |
Wang CY(王晨昱); Liu W(刘巍) ; Niu Y(牛宇); Xu P(徐鹏); Jin G(靳刚)
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| Source Publication | THIN SOLID FILMS
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| 2022-12-01 | |
| Volume | 763Pages:139578 |
| ISSN | 0040-6090 |
| Abstract | The biosensor based on imaging ellipsometry (BIE) is a practical technique to measure protein interactions such as the immobilization of biomolecules and the recognition between ligand and analyte. In this paper, to avoid the working condition optimization before each measurement, a fixed linear working condition for the silicon wafer chip is established at the angle of incidence of 75 degrees with the fixed azimuths of the polarizer and the analyzer P = 90 degrees and A = 45 degrees. Further, a quantitative model is constructed to establish the standard curve between the ellipsometric signal and the protein concentration. As a result, this model is used to deduce the dissociation equilibrium constants of three relevant blood proteins as well as their antibodies. This work bridges the gap between the biochemical demand and BIE for sensing applications. |
| Keyword | Imaging ellipsometry Biosensor Quantitative detection |
| DOI | 10.1016/j.tsf.2022.139578 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000950649200001 |
| WOS Research Area | Materials Science, Multidisciplinary ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter |
| WOS Subject | Materials Science ; Physics |
| Funding Organization | National Natural Science Foundation of China [81872584] |
| Classification | Q3 |
| Ranking | 1 |
| Contributor | Liu, W (corresponding author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China. ; Liu, W (corresponding author), Chinese Acad Sci, Inst Mech, Ctr Gravitat Wave Expt, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China. |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/91817 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Mech, Ctr Gravitat Wave Expt, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Sch Engn Sci, 19 Yu Quan Rd, Beijing 100049, Peoples R China 4.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Foundamenal Phys & Math Sci, Hangzhou 310024, Peoples R China |
| Recommended Citation GB/T 7714 | Wang CY,Liu W,Niu Y,et al. Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry[J]. THIN SOLID FILMS,2022,763:139578.Rp_Au:Liu, W (corresponding author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China., Liu, W (corresponding author), Chinese Acad Sci, Inst Mech, Ctr Gravitat Wave Expt, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China. |
| APA | 王晨昱,刘巍,牛宇,徐鹏,&靳刚.(2022).Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry.THIN SOLID FILMS,763,139578. |
| MLA | 王晨昱,et al."Approach to quantitative detection of proteins with the biosensor based on imaging ellipsometry and spectroscopic ellipsometry".THIN SOLID FILMS 763(2022):139578. |
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| Jp2022FA605_2022_App（2340KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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